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1.
Modulation of protein biophysical properties by chemical glycosylation: biochemical insights and biomedical implications 总被引:2,自引:0,他引:2
Solá RJ Rodríguez-Martínez JA Griebenow K 《Cellular and molecular life sciences : CMLS》2007,64(16):2133-2152
Glycosylation constitutes one of the most important posttranslational modifications employed by biological systems to modulate
protein biophysical properties. Due to the direct biochemical and biomedical implications of achieving control over protein
stability and function by chemical means, there has been great interest in recent years towards the development of chemical
strategies for protein glycosylation. Since current knowledge about glycoprotein biophysics has been mainly derived from the
study of naturally glycosylated proteins, chemical glycosylation provides novel insights into its mechanistic understanding
by affording control over glycosylation parameters. This review presents a survey of the effects that natural and chemical
glycosylation have on the fundamental biophysical properties of proteins (structure, dynamics, stability, and function). This
is complemented by a mechanistic discussion of how glycans achieve such effects and discussion of the implications of employing
chemical glycosylation as a tool to exert control over protein biophysical properties within biochemical and biomedical applications.
Received 15 December 2006; received after revision 28 March 2007; accepted 25 April 2007 相似文献
2.
本文给出了一个数据流图的存贮模型。并在此基础上,给出了数据流图的操作算法。可以解决SA方法中的文档管理。 相似文献
3.
间套种植对根系土壤酶及养分复合系统的影响研究 总被引:3,自引:0,他引:3
通过池栽试验研究了玉米∥蚕豆、玉米∥鹰咀豆及3种单作作物生长盛期的根系空间分布特征,以及不同种植模式对土壤酶活性、养分含量空间分布的影响。结果表明,玉米∥蚕豆和玉米∥鹰咀豆地上生物量较对照均有不同程度的提高;0cm~30cm土层中玉米∥蚕豆根干重占总根重的比例较单作对照高10 78%,玉米∥鹰咀豆较对照低5 78%;根密度在垂直方向上可划分为4个类型区,0cm~20cm为高密度区、20cm~40cm为中密度区、40cm~60cm为低密度区、60cm以下为稀密度区;土壤酶活性可划分为4个层次:亚活跃层(0cm~10cm)、活跃层(10cm~30cm)、过渡层(30cm~40cm)和稳定层(40cm以下);根密度与土壤pH、有机质含量、全磷含量、全氮含量、有效磷含量呈显著或极显著相关。表3,参3。 相似文献
4.
生物酶在羊毛织物防毡缩工艺中的应用 总被引:10,自引:0,他引:10
将蛋白酶和转移酶E2进行复配,应用于羊毛织物防缩整理,探索了最佳复配方案和工艺条件,经复合酶处理过的羊毛织物,其毡缩率可降低到1.26%,小于机可洗要求3%,应用扫描电镜观察了织物鳞片表层的破坏情况,进而了解酶作用的结果。 相似文献
5.
Aldose reductase and aldehyde reductase belong to the aldo-keto reductase superfamily of enzymes whose members are responsible
for a wide variety of biological functions. Aldose reductase has been identified as the first enzyme involved in the polyol
pathway of glucose metabolism which converts glucose into sorbitol. Glucose over-utilization through the polyol pathway has
been linked to tissue-based pathologies associated with diabetes complications, which make the development of a potent aldose
reductase inhibitor an obvious and attractive strategy to prevent or delay the onset and progression of the complications.
Structural studies of aldose reductase and the homologous aldehyde reductase in complex with inhibitor were carried out to
explain the difference in the potency of enzyme inhibition. The aim of this review is to provide a comprehensive summary of
previous studies to aid the development of aldose reductase inhibitors that may have less toxicity problems than the currently
available ones.
Received 4 December 2006; received after revision 12 February 2007; accepted 20 April 2007 相似文献
6.
李贤中 《北京工商大学学报(自然科学版)》1992,(1)
本文阐述了酶在有机溶剂中存在的两种形式:通过表面活性剂聚集体在有机溶剂中的溶解和直接以固态酶的形式悬浮于有机溶剂中.讨论了反胶团(或w/o微乳浊液)系统在酶促反应、选择性提取蛋白质等领域的应用和固态酶悬浮于有机溶剂中的性质和特点,并展望了这些系统的发展前景. 相似文献
7.
M. Kashimata M. Hiramatsu N. Minami Jr N. Minami 《Cellular and molecular life sciences : CMLS》1987,43(2):191-192
Summary An enzyme immunoassay for mouse epidermal growth factor (EGF) involving a liquid phase double-antibody system was developed. The EGF--galactosidase conjugate prepared was stable for at least 8 months. By this method, EGF was detectable at a concentration as low as 20 pg per tube. The concentrations of EGF in various tissues of mice are also presented.This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. 相似文献
8.
A. Alkofahi J. K. Rupprecht Y. -M. Liu C. -J. Chang D. L. Smith J. L. McLaughlin 《Cellular and molecular life sciences : CMLS》1990,46(5):539-541
Summary Gigantecin (I), a novel tetrahydroxy-di-tetrahydrofuran fatty acid -lactone (acetogenin), was isolated from an ethanolic extract of the stem bark ofGoniothalamus giganteus Hook. f., Thomas (Annonaceae), by means of activity-directed fractionation (brine shrimp lethality test). This new compound is extremely cytotoxic to human tumor cells, inhibits crown gall tumors on potato discs, and is active in an assay designed to detect antimitotic agents (9 ASK). 相似文献
9.
脂多糖—被动血凝试验早期诊断伤寒的价值 总被引:1,自引:0,他引:1
为评估脂多糖-被动血凝试验(LPS-PHA)早期诊断伤寒的应用价值,对病程不同的102例伤寒患者用LPS-PHA、肥达反应和细菌培养进行检测,200例非伤寒发热患者作为对照。结果显示:伤寒患者病程第一周LPS-PHA阳性率为80%(24/30),第二周达9773%(43/44);细菌培养阳性率2451%;肥达反应的伤寒与副伤寒鞭毛抗体交叉阳性率达3725%;对照组LPS-PHA有2%的假阳性。结果表明:LPS-PHA可早期、简便及快速诊断伤寒,有较高的敏感性和特异性。 相似文献
10.
Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression
vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine
deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence
analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide
by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank. 相似文献